ISO 19820:2016 specifies a method for the determination of the lethal effects of toxicants to Brachionus plicatilis after 24 h or 48 h exposure.
The method is applicable to the following:
a) chemical substances which are soluble or which can be maintained as a stable suspension of dispersions under the conditions of the test,
b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration, or centrifugation,
c) marine or estuarine waters,
d) sediment elutriates/eluates.